9.00am – 4.00pm Tuesday through Friday
Course cost $800.00. 21 places available. Discount available for UBC researchers
Due to the ever increasing interest in flow cytometry, we are hosting another 4 days course that will cover the basics through to multi-colour analysis. It is aimed at people who are new to flow or the experience user moving into the multi parameter world. wants a refresher. We will start with the basics and get more complex to allow you to walk away feeling confident that you know what to do.
Topics and talk:
- Cell proliferation, tracking and viability.
- New dyes and fluorochrome chemistry
- Panel design
- Flowjo analysis
- Instrument QC and validation
- Compensation: manual vs auto with beads or cells
- Cytof mass spectrometry and parallel technologies
- Cell sorting
Each day will start with a talk and then move into the lab to play on the instruments in groups of 3. We will start with a 6 colour panel and move onto a variety of 8-12 colour panels from different tissues; from bone marrow, spleen, thymus, to digested muscle and commercially available tubes. The end of each day will be a chance to recap on the days activity.
Each day we will perform instrument QC, experiment set up and compensation on all samples. On day 4 we will rotate around each instrument with the application specialist and cover a separate topic: cell cycle, whole blood, small particles, human blood, spectral analysis.
Minimum class size is 10, but I will open up 21-24 spaces depending on instruments available and provided by vendors.
BD LSRII (x2), BD Canto, BD Aria, Thermo Attune (x2), Beckman Cytoflex (x2)
Hopefully Sony Spectral Analyser, or Cytek Aurora and the BioRad ZE5 will be available.
- Small groups of 2-3 per instrument working through challenges.
- Instrument maintenance, trouble shooting and general up keep.
- QC choices and keeping standards high day after day.
- BD Aria and Influx; possibly more vendors well come
- Compensation 6+ colours
- Complex analysis and gating controls prior to sorting
- Cell sorting into plates, tubes and micro-tubes.