Previous Day Flow Course

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9.00am – 5.30pm Tuesday through Friday

Course cost $800.00.  Travel Bursary available to Stem Cell Network Candidates. 18 places available, with 10 places initially reserved for Stem Cell Network.

The course requires min. 10 places filled to make the course viable.  No closing date, but students accepted on first come basis and reserved upon payment.  Travel bursary are subject to SCN selection.

For the past nine years, the UBC FLOW facility has been running advanced flow cytometry courses for various research networks across Canada; for example, the Stem Cell Network and Allergen Network. We opened the course up to all students across Canada and the world.  During the advanced flow cytometry course, we provide instruction on the necessary techniques and skills to enable students to move comfortably from 3 colours on an analog instrument to handling multi-colour staining (more than 6 colours) from a variety of samples; thymus, cell lines, muscle and spleen to name a few. We focus 90% of the course on running the instruments in small groups, to enable the students to gain as much hands-on experience as possible. This allows students to come to the course with very little experience and leave with the confidence to take their flow experiments to the next level.
• Location – University of British Columbia, Vancouver, Canada  
• Registration fee includes lunches and course banquette
• University accommodation can be arranged on request

Applicants: This course is aimed at people new to flow cytometry, with no experience, through to people who are starting to consider using more colours in their experiments; or who have been self taught and missed out learning the basic fundamentals of flow cytometry. In the past, people have come with no experience and walked away comfortable doing 10 colour flow cytometry.

Course aim: To enable people to be comfortable with flow cytometry samples and fully understand the problems, fixes and trouble shooting strategies to produce high quality flow data ready for publication.

Agenda: the agenda can be changed if there is something specific that you are looking for.

9:30    Meet at the Biomedical Research Centre. group discussion to find out what people work on, interests and goals.
10:00  Introduction to flow cytometry: What you really need to know. (talk)
11:30   QC and Optimzing the system (practical)
12:30   Lunch (BD Bioscience: Multi-colour panel design)
1:30     6 colour analysis and compensation strategies: sample vs beads vs ab capture beads (practical)
3:30    Overview on compensation, colour choices and gating strategy (talk)
5:00    Finish
9:30    Morning (eBioscience: The multi coloured world, protein and mRNA staining)
10:30  QC and Optimzing the system (practical)
11:00  Sample analysis: 6 colour bone marrow and spleen, compensation controls, FMO and gating (practical)
12:30   Lunch talk ( Stem Cell Technology)
1:30     Human blood and platelet analysis (practical)
2:30    CyTOF: mass flow cytometry (talk)
5:00    Finish
9:30    Overview of previous day (talk)
10:30  QC and Optimzing the system (practical)
11:00  Sample analysis: muscle digest and  bone marrow and spleen, compensation controls, FMO and gating (practical)
12:30   Lunch talk (Beckman Coulter: Intracellular Signalling)
1:30     Cell cycle, intracellular and Cytokine assays (practical)
3:30    Flowjo analysis (practical)
5:00    Finish
9:30  Cell Sorting Theory (talk)
11:00   Rotations through:
1) Cell Sorting (practical) Miltenyi Cell separation  technology.
2) Life Technolgies Attune analyser
3) Sony Cell Sorter
4) Beckman Coulter analyser
5) Miltenyi Cell separation  technology.
3:30    Finish
Topics covered:
• Instrument QC and optimization.
• Compensation in a multi-colour world.
• Fluorochrome pairing and making the most of the colours.
• Apoptosis, CBA, BrdU, Cell Cycle assays.
• Trouble shooting different samples/tissues.
• 8+ colour analysis.
• Introduction to cell sorting.*
• Auto-fluoresence – understanding what is real.
• Producing good flow data and avoiding the simple mistakes.
• Fundamentals of cell sorting: the key things to remember.
• Multicolour panel design.
• CyTOF principles and design
• Talks by:
  • BD: Panel design,
  • Miltenyi: Cell separation techniques and applications,
  • eBioscience: Fluorchrome properties,
  • Flowjo analysis class,
  • Fluidigm: CyTOF, mass spectrometry for flow cytometrist.
* specialized training in cell sorting can be arrange for time before or after the course date, using the BD Influx, Aria or DiVa cell sorters. Only the basics can be covered in a group session. Instruments: BD Aria, BD LSRII, BD Canto, BD Fortessa.