Covid: phase 1 operation

Operation of UBCflow core during Covid19

The UBCflow core is based out of two buildings and both locations will have very specific protocols that will need to be followed to provide a safe opening.

BRC researchers are expected to use the BRC flow instruments. LSC researchers are expected to use the LSC flow instruments. If you are from a different location we will need to make special arrangement through the facility to gain access to the buildings.

Due to a max room/building occupancy and tracing ability, all other researchers will need to book through the facility via emails. Building access maybe heavily restricted. Due to this, it is highly recommended that all non-BRC researchers who use the flow facility move to the LSi, as it is easier to manage access control and tracing. At all time we need to know who is in the UBCflow area, what they used and when they used it. This is currently managed through the online flow scheduler.

The UBCflow LSi facility is located in an isolated wing of the LSi, with secure card access and very limited foot traffic.  During Phase 1 we will limit one user to each instrument and each room. If we need to handle a larger capacity, we have the option to move some instruments around. The max room occupancy will be enforced via the online schedule: when you book the instrument, you book the whole room. 

  • 5 flow cytometers at the LSC: (Canto, Attune, LSRII, LSR561 and Cytoflex LX) located in three zones:
    1. Zone 1 (Canto and LSRII)
    2. Zone 2 (Attune and LSRII-561)
    3. Zone 3 (Cytoflex)
  • 2 flow cytometers at the BRC: (LSRII and Cytoflex LX)
  • 5 cell sorters at the LSC: Room 1 (two Influx and Astrios) Room2 (Aria and Jazz)
  • 1 cell sorter at BRC: (Astrios)

Hours of operation:

The facility remains accessible 24/7 with people booking the instruments using the online schedule and monitored by staff.  The facility will have personnel at UBC between 8am to 10pm to assist people, operating a double split shift. We appreciate that the access hours to other labs and resources will me limited, so hopefully the extended UBCflow hours will assist.

  • Andy Johnson will cover 8am (instrument clean and room decontamination), through to the 3pm, with a flexible end time depending on demand.
  • Justin Wong will cover 1pm to 10pm (end of day instrument clean and room decontamination), with a flexible end time depending on demand.
  • These hours will cover the time frame when we expect sorting requests.
  • The facility will support work 24/7 with a flexible schedule in place.

Cell sorting, assisting users or fixing instruments:

Cell sorting and support to users will comprise of two overlapping shifts (9am to 9pm ~ flexible).  When Justin arrives for the second shift, I will move out of the sorting facility at the LSC and move to the BRC facility or the LSC overflow facility: a second cell sorting room at the LSC housing the Aria and BD Jazz cell sorter.

There are times when we need to be close to users at the instrument, for example; when we are trouble shooting samples or setting up for cell sorting populations.  UBCflow staff will wear a lab coat, gloves and mask at all times when interacting with users or outside the cell sorting room; this will be kept to a minimum.

Minimize complex sorting where we need to optimise gating and sorting strategy. Only experienced users may use the equipment during Phase 1 as not training will be provided. If the experiment is in the priority list, the UBCflow facility staff will run the experiment for you.

  • Most cells sorts are now routine and we have gating and objectives pre determined.
  • This allows most users to drop off and pick up their samples from the door without entering the cell sorting room.
  • Users entering the cell sorting room, require lab coat, gloves and mask.
  • Only two people maybe in the cell sorting room.

SOP for accessing facility:

  1. At 9am all instruments and surfaces will be wiped down and turned on and repeated at the end of the day.
  2. All uses must wear clean gloves or wash hands upon entering and exiting the facility, to operate and use flow cytometers safely.
  3. Scheduling of a single instrument, with one operator. Room max occupancy one.
  4. Wipe down all work areas and touch surfaces with ethanol before after use.
  5. Book using the online schedule and provide, name and contact details so you can be traced.
  6. Do not enter the room until the first person has left. 
  7. Allow 15mins between bookings to allow for air circulation.

Staff sickness:

In the event that myself or Justin get sick and have to self isolate, we will request the help of additional support to do the room cleaning at the start and end of each day, as this will allow the single operator to focus on cell sorting.

UBCflow Cell Analyser Rooms LSi

  • You need approved card access to the building or arrange a meeting with the UBCflow staff.
  • Do not enter the room unless you have made an online booking.
  • Do not enter if there is another person in the room.
  • Knock and wait outside the room.
  • Allow 15 minutes between users.
  • Wear gloves and mask inside the room.
  • Spray/wipe down the computer and all touch points.

UBCflow Cell Analyser Rooms BRC

  • You need approved card access to the building or arrange a meeting with the UBCflow staff.
  • Do not enter the room unless you have made an online booking.
  • Do not enter if there is another person in the room.
  • Knock and wait outside the room by the stairs.
  • Allow 15 minutes between users.
  • Wear gloves and mask inside the room.
  • Spray/wipe down the computer and all touch points.

UBCflow Cell Sorter Rooms

  • Do not enter the room unless you have made an online booking.
  • Call 15mins before you come to the facility.
  • Wait outside the room at all times, until invited in.
  • Place sample box immediately on the left as you enter the room.
  • Collect samples on table outside the room area.
  • If you need to discuss gating, you must wear gloves and mask to enter.